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12 (11), 639665 (2022)

Lentigo maligna has a comparatively low rate of transformation to invasive melanoma (under 5%)

The resin-bound protein was washed successively with Buffer A and finally eluted with the elution buffer containing a gradient increase in the concentration of Imidazole (50 mM Tris-HCL, pH 7.5, 500 mM NaCl and 50 mM/100 mM/250 mM Imidazole)

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