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Buffer B concentration was brought to an initial 2% concentration in the next 0.5 and the column was equilibrated at this buffer condition for 1.5 minutes before the next sample injection

Furthermore, a similar results were reported in a study of individuals with or without oligozoospermic which showed no significant association between DNA methylation in spermatozoa and the MTHFR C677T genotypes although a trend for higher incidence of methylation alterations in severe oligozoospermic infertile men with CT genotypes were observed 34 , suggesting that a much more complicated or indirect interactions between MTHFR polymorphism and methylation are involved

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