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Such Gln27 mutations, along with Arg16 mutations in adults, can result in an unfavourable cardiovascular profile (7981)

Preparation of cell lysates and immunoblotting To verify the knockdown of TMEM55B and TBC1D9B , cells were lysed on ice in PBS with 1%(v/v) Triton X-100 and 1x cOmplete Protease inhibitor cocktail (Roche) for 60 min, followed by 3x 12 s sonification with a Branson Sonifier 450 (Emerson Industrial Automation)

Coronary microvascular rarefaction and myocardial fibrosis in heart failure with preserved ejection fraction

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