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Consequently, changes to this 9-mer region, particularly those that abrogate homology with other peptides in the human proteome, might be detrimental to homology-induced tolerance and lead to increased immunogenicity risk ( Selection of impurities for in vitro studies The 34 observed impurities evaluated in silico included impurities resulting from amino acid deletions, amino acid duplications, side-chain modifications (including oxidation, succinimide formation, dehydration, acetylation), N- and C-terminal truncations, and N-terminal extension and modification ( Figure 4 )

Acknowledgements Author gratefully acknowledges Dr.S.G.Bhat, Head, Department of Biochemistry and Nutrition and Dr.V.Prakash, Director, CFTRI, Mysore for their encouragement in preparing this review

Cell Physiol Biochem (2014) 33:107586