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Equal concentrations of total RNA (10 g) were size-separated on 1.2% agarose-2.2 M formaldehyde gels and blotted onto Hybond N+ membranes (Amersham Biosciences)

Supplementing can help replenish these levels, though effectiveness often depends on the supplement's absorption and bioavailability

The increased intracellular ROS production in PD PBMCs compared with matched controls does not occur at the expense of glutathione reductase [124] but is dependent on the daily levodopa dosage, suggesting that levodopa may help modulate the redox status of PBMCs [124]

The effects of GIP on glucose metabolism are an area of ongoing investigation, which will hopefully be accelerated by the development of a specific GIP receptor antagonist (103)