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After embryo culture, the rates of blastocyst development from cleaved two-cell embryos in each group were microscopically observed and calculated as the percentage of blastocysts/cleaved two-cell embryos

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After electrophoresis, the gel was stained with a staining solution [0.1 % coomassie brilliant blue (CBB) R250, 45 % methanol, and 10 % acetic acid] for 2 h