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After this point, both peptide degradation and bacterial contamination risk increase beyond acceptable thresholds, even with bacteriostatic water as the diluent
Unapproved Drugs and Devices. FDA.gov
Highlighting the specificity of TGM1, involucrin contains 39 repeats of a 10 amino acid sequence, with each containing three glutamine residues (Eckert and Green, 1986), but only one glutamine residue within this 585-amino acid proteinin the sequence ELPEQ Q VGQP (reactive Q is in bold)is reactive in vitro unless the protein is proteolytically degraded (Simon and Green, 1988)
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