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Once you are done washing with these buffers, you will immediately rinse with a few column volumes of dIH 2 O followed by a few column volumes with binding buffer, or some kind of buffer with a neutral pH around 7 to 8
emphasized that the black box nature of these models, i.e., their lack of interpretability, limits their clinical acceptance, necessitating explainable AI (XAI) techniques to enhance trust [173,174]
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Several factors may explain this outcome
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