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Methods: Gastrocnemius injury in NASH mice fed with a choline-supplemented amino acid diet were accessed via qPCR, histological staining and electron microscopy

The supernatant was transferred to a 96-well plate and supplemented with Daily Assay Reagent (DNTB (5,5-dithios-(2-nitrobenzoic acid)) and NADPH (nicotinamide adenine dinucleotide phosphate) in GSH stock buffer)

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The enhanced surface targeting of LaccID was further confirmed by antibody staining, with and without membrane permeabilization, followed by FACS analysis (Supplementary Fig