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Throughout the day, the body produces unstable molecules called free radicals as a byproduct of normal metabolism, exposure to environmental pollutants, and stress

In addition, studies have shown that glial cells can influence vascular dynamics in the retina, which is essential for maintaining adequate blood flow and nutrient delivery to RGCs (171, 175)

Thus, being able to measure all forms of GSH in one well significantly reduced the sample volume required for our experiment and mitigated inter-plate variation as a confound in data analysis