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Reconstitution technique may have compromised potency

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Media and cell AAVs were combined and loaded onto an iodixanol (OptiPrep Density Gradient Medium, MilliporeSigma, D1556250) gradient at 15%, 25%, 40% and 60% in 1 gradient buffer and subjected to ultracentrifugation

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Academy of Otolaryngology-Head and Neck Surgery-Bulletin(12):45, 2010

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