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In contrast, another demonstrated that macrophages, during the efferocytosis process, present a transient increase in glycolysis ( ~ 1h-6h), that is dependent on rapid post-translational mechanisms with increased expression of GLUT1 on the cell surface and glucose uptake, in a process that involves the activation of the glycolytic enzyme fructose-2,6-bisphosphatase 2 (PFKFB2)

For frozen sections, 4% paraformaldehyde-fixed brains were mildly shaken in 20% sucrose solution for 6 h and then 30% sucrose solution overnight at 4 C

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Cell 127 , 12951297 (2006)