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Assessment points: Week 4: Initial assessment of response

Crystal violet staining revealed that the number of viable GC cells was greater in the simvastatin+oe-PD-L1 group than in the simvastatin stimulation groups of GC cells when cocultured with activated CD8 + T cells (Fig

Each sample was resuspended in 20 l of LC/MS grade water and 10 l per sample was injected into a 5500 QTRAP, a hybrid triple quadrupole/linear ion trap mass spectrometer, using a quantitative polar metabolomics profiling platform with selected reaction monitoring (SRM) that covers all major metabolic pathways