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These synergy studies established the mechanistic principle underpinning the GHRH analogue + GHS-R1a agonist combination format demonstrating that dual-pathway somatotroph co-stimulation is not merely additive but produces signal amplification through convergent intracellular mechanisms

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Knock-in was confirmed via junction PCR and digital PCR targeting the precise knock-in site, showing approximately 3% knock-in efficiency in the Donot+Cas9 group (Fig