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Research suggests that fracture patients need 1.5 to 2.0 grams of protein per kilogram of body weight per day during active healing, significantly more than the general recommendation of 0.8 grams per kilogram

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The pituitary gland and hypothalamus were collected and frozen for analysis of mRNA expression of GH, GHRH, GHSR, SST, and SSTR by real-time polymerase chain reaction (RT-PCR) and protein expression by Western immunoblotting