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Its absence doesn't eliminate bile production but changes how it flows continuously rather than on-demand, affecting fat metabolism

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Western blot Proteins were extracted from Huh7 or Hep3B cells and separated using 10% sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, cat