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Furthermore, based on the Kd (100 M ) and saturating GABA concentration (1 mM ) for GABAa receptor binding and desensitization [251,252,253,254] and assuming a stoichiometry of 2 for GABA co-released with copper [112] one could also conlude that a copper concentration of 100 M can exist transiently within the synaptic cleft

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GCL-catalyzed conjugation of the -carbonyl group of glutamate with the amino moiety of cysteine is the rate-limiting step in glutathione synthesis