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Metabolite measurements using LCMS For aqueous metabolites extraction, serum (5 l) was mixed with 150 l of extraction solvent (40:40:20 methanol:acetonitrile:water, v:v:v) at 20 C, vortexed and immediately centrifuged at 16,000 g for 10 min at 4 C
This approach requires precise timing, excellent technique, and careful carbohydrate management since youre driving significant glucose uptake across multiple tissues simultaneously
Vice versa, laparoscopy may be omitted if during inguinal exploration a nubbin is found and removed [23]
[DOI] [Google Scholar] 58.dos Santos M., da Silva Jnior F.M.R., Muccillo-Baisch A.L
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