HC neutrophils were cultured in the presence of HC or SLE serum with or without the addition of Cl-amidine (Cl) (peptidyl arginine deiminase 4 (PAD4) inhibitor, 100 M), or LPX-1 (1 M) for 4 or 16 hours and NETs were assessed in SYTOX Green+ cells based on morphology (n = 6)
Briefly, it covered: a) removal of low-quality precursors and contaminant protein groups, b) precursor intensities normalization by loessF algorithm c) protein group MaxLFQ intensities calculation and log2 transformation, and d) differential expression analysis using LIMMA statistical test
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The Physiology of Glucagon-like Peptide 1 [La fisiologa del pptido similar al glucagn tipo 1]
