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Diarrhoea increases fluid losses through the gastrointestinal tract

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Think of it as both a copper delivery system and a gene modulator : Copper is essential for enzymes that build collagen, defend against oxidative stress, and produce cellular energy

The deviation from linearity is probably due to geometric constraints in the roGFP barrel similar to those described for roGFP1-iR which had been expected to be less reducing than roGFP1-iL due to stabilization of the thiolate anion by the introduced adjacent basic arginine residue (Lohman and Remington, For live cell measurements biosensors should ideally exhibit a strong preference for one specific analyte