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Common symptoms can include: Extreme fatigue Lethargy Loss of appetite headaches Feeling faint or dizzy Breathlessness Headaches Heart palpitations Tinnitus Additional symptoms of B12 deficiency can include psychological problems such as low mood, irritability, depression, cognitive changes, or blurred memory

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In addition, HILIC is usually run with MS-compatible solvents and buffers, which can provide a solution when IP-RPLC conditions cause ionic suppression , further improving sensitivity during LC-MS analysis of low-level impurities