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For luciferase reporter assays, cells were transfected with either a psiCHECK2 plasmid containing the human SIRT1 3 UTR sequence (VectorBuilder) or a pRF plasmid containing the HK2 5 UTR sequence

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23,24 Elevated postprandial GLP-1 concentrations may be influenced by mechanical stimuli such as gastric distension, 3,25 which also activates GLP-1-expressing neurons in the nucleus tractus solitarius (NTS)

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