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RNA extraction, RT-qPCR and RNA-Seq Total RNA was extracted from Hs 578 T cells using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany), according to the manufacturers instruction

When administered by the experienced professionals at Skin Studio Toronto, these injections not only bolster your bodys natural detoxification processes but also help inhibit melanin production (the pigment responsible for dark spots, melasma, and sun damage)

For LDH assays, 60 l of supernatant from wells containing BMDMs was collected into 96-well plates and LDH assay buffer was added according to manufacturers instructions (Promega CytoTox 96 Non-Radioactive Cytotoxicity Assay, G1780)