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The extract was then centrifuged by using Hermle Centrifuge Z216MK at 15,000 rpm for 5 min at 0 C

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Pain 155(2):269274 Ben Aissa M, Tipton AF, Bertels Z, Gandhi R, Moye LS, Novack M, Bennett BM, Wang Y, Litosh V, Lee SH, Gaisina IN, Thatcher GR, Pradhan AA (2018) Soluble guanylyl cyclase is a critical regulator of migraine-associated pain