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The final purified samples were desalted into phosphate buffer saline (PBS) for later tests and separated on a gel with a concentration of 12.5% SDS-PAGE

It is a nonselective melanocortin receptor agonist, and its therapeutic effects are believed to arise mainly through central MC4R-mediated signaling , with possible contributions from MC3R and other melanocortin receptors

The results of the study showed that intravenous magnesium could relieve acute attacks between 15 min and 24 h after infusion, and oral magnesium significantly reduced the frequency and intensity of migraine attacks (187)

Plant Physiol 141:373378 Trotta A, Rahikainen M, Konert G, Finazzi G, Kangasjrvi S (2014) Signalling crosstalk in light stress and immune reactions in plants