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All mice were housed under a 12 h light/dark cycle, with the temperature consistently maintained between 18 C and 23 C and the humidity kept at ~50%

However, one of the most notable differences in formulation is between the usage of live and pasteurized Akkermansia

Therefore, based on our observations and those found in the literature, we suggest that the daily intake of energy drinks should not only not exceed the safety limits for caffeine established by European and American regulatory authorities, but should be even lower