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Handling with Cell Culture Applications For cell culture work, all Glow Stack peptides should be: Reconstituted with sterile, endotoxin-free water or PBS Filter-sterilized through a 0.22 m syringe filter before adding to culture media (if not already in a sterile solvent) Added to cell culture media at the desired final concentration immediately before use, not stored diluted in media long-term Kept at 4C or on ice during cell culture work to minimize degradation between uses Freeze-Thaw Cycle Management Each freeze-thaw cycle causes some degree of peptide degradation particularly through aggregation and oxidation at ice crystal surfaces

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There's no real debate here

Michael White, head of the department of pharmacy practice at the University of Connecticut