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The extraction solvent consisted of 80% methanol and 20% aqueous solution (pH 7.00) containing 10 mM ammonium formate (Sigma-Aldrich, 70221-25G-F) and 25 mM NEM (Thermo Scientific, 040526.06)

The hydrophobic fraction of SOM was assumed to comprise a small fraction of total SOM (57% by mass