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When they do come to the end of their useful life, they need to be discharged and properly disposed of

Such disorders may be caused, for example, by a decrease in synaptic connectivity and/or neuron density due to a variety of factors

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2.3 MTS assay GDC was dissolved in DMEM D1145 and filter sterilized using 0.22 m PET syringe filters, (A16532-GUK, Misart Engineering Ltd, New Zealand), to a concentration of 0.5 to 5 mM