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TUNEL assay On experimental day P38, pancreas samples were fixed in 2% w/v paraformaldehyde for 2 h and processed for TUNEL assay, which was performed using the One Step TUNEL Apoptosis Assay Kit (Beyotime, Shanghai, China) according to the manufacturers instructions

In this study, we hypothesized that FOXO4-DRI treatment could remove the senescent cells in the expanded chondrocytes, thus enhancing their potential in generating high-quality cartilage

-6 PUFA typically come from animal sources (meat, especially chicken, and eggs) and to a lesser extent from plant sources (soy, sunflower seeds and oils), while fatty fish and fish oils, leafy green vegetables, vegetable oils, nuts and seeds are rich in -3 PUFA [120]