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In high concentrations, however, some amino acids can be toxic to certain cells, as observed with Glu toxicity in neurons [45] and Cys toxicity in many types of cells, including neurons and hepatocytes

In women, this can lead to problems with ovulation, irregular periods, or conditions like polycystic ovary syndrome (PCOS)

An aliquot of 20 l of the third-round virus mixture was used to infect 500 ml (SFM900-II medium with 5% FBS, Thermo Fisher) of Sf9 cells (1.5 10 6 /ml) for protein expression in an orbital shaker (Shanghai Zhichu Instrument) at 27 C and 200 rpm