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Mass cytometry Heavy-metal conjugation of antibodies Antibodies were conjugated to heavy-metal ions with commercially available Maxpar (Fluidigm) or MIBItag (Ionpath) reagents according to manufacturers instructions, and as previously described 30 (see Supplementary Data 2)

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Although the paper does not outline specific criteria for identifying patients who might deteriorate significantly, some combination of dose ingested, time of ingestion, specific form of VPA involved (long-acting preparations pose an increased risk of continued delayed absorption), serum VPA level on presentation, and symptoms should be considered