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X-ray photoelectron spectroscopy (XPS) was performed on a Thermo ESCALAB 250Xi spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) to investigate the elemental composition and chemical states of the samples

Amplification and sequencing of Core and NSB regions For HCV genotyping, total RNA was extracted from 140 L of plasma using QIAamp Viral RNA Mini Kit (Qiagen, Courtaboeuf, France), according to the manufacturers instructions, following by amplification of Core and NSB regions using nested polymerase chain reaction (nested-RT PCR)

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In addition to detoxification, glutathione is involved in DNA synthesis and repair, protein synthesis, and immune system support