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Cells were grown in GSH-free DMEM medium (Hyclone, Logan, UT, USA), supplemented with 10% fetal bovine serum (FBS, Hyclone), 2 mM glutamine (Sigma-Aldrich), 100 U/mL penicillin (Hyclone), and 100 ug/mL streptomycin (GenDEPOT, Barker, TX, USA) at 37 C in a humidified atmosphere with 5% CO 2