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Never leave it out during injection preparation longer than necessary
It could, but it definitely shouldnt be
Baseline: CBC, B12, folate, iron studies Repeat B12 levels 1 month after loading dose Annual checks for chronic therapy Monitor potassium during initial treatment Neurological exams for neuropathic symptoms B12 injections can "mask" megaloblastic anemia from folate deficiency while allowing neurological damage to progress
coli cells (100 mL) were collected (6000 rpm, 4 C, 10 min) and resuspended in 5 mL of lysis buffer containing 8 M urea, 4 % (w/v) CHAPS, and 1 % (w/v) DTT and sonicated on ice for 90 cycles (a working period of 3 s in a 6 s interval for each cycle) at a power output of 200 W by an ultrasonic disruptor (JY92-II, Scientz Biotechnology Co., Ningbo, China)
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