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It was with the five-milligram dose, which is one of the lower doses of tirzepatide, Skelley says

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The best binding was observed for the histidine containing peptide as expected, whereas slightly weaker affinity was observed for amide (Q, N) or amine (K) containing side chain, whereas carboxy (D,E), thioether (M) or aromatic (F,Y,W) functional groups hardly enhanced affinity compared to the single histidine containing peptide (Fig