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Onyimba F, Crowe SE, Johnson S, Leung J

Immunostaining was performed by seeding cells on glass coverslips, fixing with paraformaldehyde, permeabilizing with Triton X-100, and then incubating with primary anti-NF-B p65 antibody, followed by Alexa Fluor 546-conjugated secondary antibody

Since the fusion of an albumin-binding moiety to proteins instead of directly fusing biologics to albumin has several advantages in terms of production cost and safety 49 , the use of a binding protein known as DARPins has been decided upon in this work

Rather than following traditional dosing protocols, patients and physicians are exploring whether smaller amounts can still provide meaningful benefits while minimizing potential side effects