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MDA formation is the result of decomposition of the unstable peroxides derived from polyunsaturated fatty acids TBARS may not be an effective marker for GSH

Gadkar K, Pastuskovas CV, Le Couter JE, Elliott JM, Zhang J, Lee CV, et al

Cell cultures Cortical neurons in primary culture were prepared from fetal Wistar rats (E16), seeded at 2.510 5 cells per cm 2 in 6- or 12-well plates previously coated with poly-D-lysine (15 g ml 1 ) in DMEM (Sigma, Madrid, Spain) supplemented with 10% (v/v) fetal calf serum (Roche Diagnostics, Heidelberg, Germany)

Mammals possess five HK isozymes, namely HK1, HK2, HK3, glucokinase (GCK), and hexokinase domain-containing 1 (HKDC1), with HK2 being the most extensively studied in glycolysis ( Figure 1 )