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doi:10.1136/bmjophth-2022-001154 29

Abstract The control of cysteine reactivity is of paramount importance for the synthesis of proteins using the native chemical ligation (NCL) reaction

The characteristic pattern in S2 was the successive positive palatability contrast effect a dramatic increase in food intake in the b S1 v S2 meal schedule condition compared to all the remaining three meal schedule conditions [34]

Reconstitute with sterile bacteriostatic water or appropriate laboratory buffer Add solvent slowly along the vial wall to minimize foaming Gently swirl until the peptide is dissolved Store reconstituted solutions at 3646 F (28 C) Prepare aliquots where appropriate to reduce freezethaw cycles Careful reconstitution helps maintain peptide stability and functional integrity