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The binding constant of GHK for copper (log10 = 16.44) slightly exceeds that of albumin (log10 = 16.2), enabling the peptide to acquire Cu2+ from plasma proteins and deliver it to cells while silencing coppers potentially damaging redox activity (Pickart, 1977)

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A rotary tattoo machine (Cheyenne, by MT.DERM GmbH) with a 27-needle cartridge was then used to drive the solution into the scalp at 70 Hz oscillation (producing 1,890 perforations per second) with a 2 mm needle exposure depth