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Gene expression changes also involved pathways linked to autoimmune diseases, cancer, and major depressive disorder, indicating that even brief circadian disruption can trigger systemic inflammatory responses with potential long-term health consequences 146

Individual colonies were expanded in 12 well plates, genomic DNA was extracted with PureLink Genomic DNA Mini Kit (Thermo Scientific, K182002), as well as protein lysates were prepared in RIPA buffer (50 mM Tris-Cl, pH 8, 150 mM NaCl, 5 mM EDTA, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, supplemented with protease inhibitors, Sigma, P8340)

pylori infection is complicated and not simply linear