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If you have not lost at least 5% of your starting body weight after twelve weeks on the full maintenance dose, most clinical guidelines suggest Contrave may not be the right fit, and it is worth revisiting your plan with your physician

Total protein of the cells was extracted and reduced in the presence of 100 mmol/L DTT for 5 min on the boiling water bath, and subsequently alkylated with 50 mol/L iodoacetamide for 30 min at room temperature in the dark