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Briefly, purified SAPK2-His and ABI5-GST proteins were incubated at 30 C for 30 minutes in kinase reaction buffer containing 500 mM HEPES-KOH (pH 7.5), 150 mM NaCl, 100 mM MgCl 2 , 1 mM EGTA, 1 mM ATP, 10 mM DTT

[7] In practice, some protocols use 1 mg IM monthly for maintenance once levels are stabilized

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