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Reaction products from a standard MSA-DH assay were fractionated by preparative HPLC separation (compare Figure 4A) and the malate containing fraction was subjected to enantiomeric separation on a Chirex ligand exchange phase 3126

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[DOI] [PMC free article] [PubMed] [Google Scholar] 87.Mulliez E, Ollagnier S, Fontecave M, Eliasson R, Reichard P