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2014 Jul 14;9(7):e101956

Changes in HbA1c levels from baseline to week 26 was the primary efficacy endpoint measure by the research team while the secondary efficacy endpoints measure at week 26 included the proportion of patients achieving HbA1c target levels of less than 7.0%

Feng X-H, Liu X-M, Zhou L-H, Wang J, Liu G-D

The samples were precipitated again by methanol-chloroform-water and then resuspended with 1 Laemmli buffer without DTT, boiled at 95 C for 3 min, separated by SDS-PAGE, and analyzed by immunoblotting