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SF3B1 in-vitro binding assays 1 g of purified GST-tagged SF3B1(1463) in 48 l of kinase buffer (20 mM Tris-HCl, pH=8.0, 5 mM MgCl 2 , 2 mM DTT, 2 mM ATP) was incubated with 12 ul flag-tagged CDK11 kinase (immunoprecipitated from HEK293 cells) or with 12 ul of elution buffer (20 mM Tris-HCl (pH=7.5), 150 mM NaCl, 1 mM DTT)