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[2] As GLP-1 binds to GLP-1 receptors expressed on the pancreatic cells, the receptors couples to G-protein subunits and activates adenylate cyclase that increases the production of cAMP from ATP

iGL cells were exposed to different mediums: iGL cells culture medium (Cell only), iGL culture medium supplemented with synthetic Exe4 peptide (Exe4 solution) and with scExe4 peptide secreted from Hepa1-6 and HeG2 cells transfected with the scExe4 expression plasmid (scExe4_Hepa1-6 and scExe4_HepG2, respectively)