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This study, along with other studies involving the PLID, will have a significant impact on the treatment of patients with type-2 diabetes in the future

Metformin is commonly used as a first-line treatment along with diet and exercise for those just diagnosed with type 2 diabetes

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Then, these permeabilized cells were stained with 25 g/ml PI for 10 min and finally analyzed using a BD FACSVersse TM flow cytometer (Becton, Dickinson and Company, San Jose, CA) with an argon laser tuned to the 488 nm line for excitation