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Cells were then lysed in 80 l surfactant cocktail data 64 with 4 l benzonase added to each plate at 5 U l 1 (diluted 1:50 from 250 U l 1 stock) right before lysis, to prevent benzonase inactivation by the lysis buffer

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Cells expressing the SCoV M pro also became apoptotic, which is a known consequence of NF-B activation, especially in combination with a high burden of ROS (19), which is even more likely to occur if other antioxidant proteins are also degraded, as discussed below