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Biohacking, which is the use of nutrition, technology, pharmacology, and self-experimentation to optimize biological performance, overlaps with longevity science, the quantified self movement, and bodybuilding culture
For quantitative analyses, ratio of cells positive for nuclear -catenin staining to total number of cells was counted as pixel intensity ratio positive for nuclear localization using Image J (NIH, Bethesda, MD, USA)
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